Optimization of Flow Injection (FI) – Spectrophotometry for Hydroquinone Analysis

Authors

  • Muhammad Iqbal Fahmi Chemistry Department, Faculty of Science, Brawijaya University, Jl. Veteran Malang 65145 Indonesia Author
  • Hermin Sulistyarti <ol><li>Chemistry Department, Faculty of Science, Brawijaya University, Jl. Veteran Malang 65145 Indonesia</li><li>Research Center for Low Cost and Automated Method and Instrumentation Analysis (LCAMIA), Brawijaya University, Indonesia</li></ol> Author
  • Ani Mulyasuryani Chemistry Department, Faculty of Science, Brawijaya University, Jl. Veteran Malang 65145 Indonesia Author

Abstract

Hydroquinone is one of phenolic compounds used in various cosmetic products for skin lightening as it can inhibit the tyrosinase enzyme in producing melanin. However hydroquinone is classified as toxic compound therefore several countries such as Africa, Canada and Indonesia prohibited hydroquinone utilization in cosmetics. This Research was focused on the development of method for hydroquinone analysis using flow injection (FI) combined with spectrophotometry based on the reaction of hydroquinone with phloroglucinol in alkaline condition producing orange complex detected at wavelength 483 nm. The FI ​​method optimized based on operational factors and chemical factors in order to achieve sensitivity. Maximum sensitivity of FI method was achieved under operational condition of 100 μL sample volume, 100 cm mixing coil 1, 50 cm mixing coil 2 and 2,8 mL mins-1 with chemical condition of 0,005 mol L-1 NaOH and 0,001 mol L-1 phloroglucinol. Under these obtained optimum condition, the proposed method showed linearity in the range concentration 2 – 50 mg L-1 and the method has been applied to cosmetic sample with acceptable recovery.

Keywords: Hydroquinone, Flow Injection, Phloroglucinol, Optimization

Published

2018-09-01

Issue

Vol. 1 No. 1 (2018): The 1st ICoSTES 2018

Section

Basic Sciences