Determination of cadmium in gill, liver, and muscle of fish with solid phase extraction (SPE) based on the ionic imprinted polymer (IIP).

Abstract

An ion-imprinted polymer (IIP) material with 8-hydroxyquinoline (8HQ) (the complexing ligands), methacrylic acid (MAA) (the monomers), ethylene glycol dimethacrylate (EGDMA) (the crosslinker agent), benzoyl peroxide (the initiator), ethanol-acetonitrile (2:1) (the porogen), and cadmium (template) has been synthesized. The IIP was used as an adsorbent for the determination of cadmium by solid phase extraction – flame atomic absorption spectrometry (SPE-FAAS). Variables affecting the SPE process, such as pH, elution volume, and flow rates of the sample, were evaluated. This method was applied to determined of cadmium in gill, liver, and muscle of Oreochromis niloticus. The optimized procedure consists of a sample loading (sample pH of 6) through IIP-SPE columns containing 100 mg of the synthesized IIP at a flow rate of 0.5 mL min^-1. Cadmium can be eluted completely by passing 2 mL of 1 mol L^-1 HNO₃. The recovery of the method were 95.61, 94.60, dan 95.52 % for in gill, liver, and muscle, respectively.

Keywords: cadmium, SPE, IIP.