Histochemical analysis Oleuropein and in silico observation on secondary metabolites of olive leaves (Olea europaea L.)

Abstract

So far, it has known the benefits of olive oil extracted from the fruit. But apparently there are other parts from the olive plant (Olea europaea) which has many benefits, especially for health, and that one part is leaf. Oleuropein belongs to the iridoid group which has antibacterial, antiviral and antifungal activity, and is useful for resisting various types of internal infections. Oleuropein can also increase the production of thermogenin inside the body, thermogenin is a compound that can burn fat effectively. In a recent study, oleuropein can be useful for lowering blood pressure. Studies on animals show that olive leaf extract can prevent nerve damage, thus, it is good for preventing strokes. This study aims to determine the important compounds oleuropin of olive oil found in olive leaves.

Discovery of secondary metabolites in olive leaves was carried out by histochemical analysis by giving certain reagents to epidermal tissue on the leaves seen with an Olympus DP73 microscope. After discovering the metabolite content, the In silico method was observed using the computer simulation method through the PubChem data base (https://pubchem.ncbi.nlm.nih.gov/ ). The specifications of the chemical elements found in olive leaves (Olea europaea L) will be found out on PubChem. Metabolite compounds found in a plant species were carried out with the KNApSAck-3D database (http://kanaya.naist.jp/knapsack_jsp/top.html).

The results showed that histochemical analysis of the 5 positive secondary metabolites contained in olive leaves were alkaloids, terpenoids, flavonoids, lipophilic. While with in silico observation, the secondary metabolites contained in olive leaves can be seen primarily in the form of 3D structures. Specific compounds found only in olive leaves are 1-Acetoxypinoresinol, oleuropein, Cis-3-Hexenyl acetate, Cis-2-Hexen-1-ol and  Methyl nonanoate. It showed that the oleuropein compound is found in olive  leaves.

Keywords: Olive (Olea europaea L.), histochemistry, In silico