POTENTIAL KOMBUCHA Annona muricata L. and Clitoria ternatea AS AN ANTIBACTERIAL of Escherichia coli

Abstract

POTENTIAL KOMBUCHA Annona muricata L. and Clitoria ternatea AS AN ANTIBACTERIAL of Escherichia coli

Kalyana Palupi, M. Rizal Fauzi, Dinda Dwi Anggita, Dini Sri Damayanti*

Faculty of Medicine, University of Islam Malang

ABSTRACT

 

Introduction: Kombucha Annona muricata Linn and Clitoria ternatea contain organic acids, phenolic compounds, steroids/terpenoids, tannins, alkaloids and flavonoids which have the potential as antioxidants, anti-inflammatory and antimicrobial. The purpose of this study was to determine the potential kombucha Annona muricata Linn and Clitoria ternatea as an inhibitor of the growth of Escherichia coli.

Method: The preparation of Annona muricata Linn and Clitoria ternatea kombucha begins by boiling 1 L of water until it boils, then adding 100 grams of sugar and 2 grams of A. muricata Linn and Clitoria ternatea powder. Then cooled and put in a closed jar, then added 24 grams of SCOBY and covered with tissue paper. The fermentation process was carried out for 7 days. The Zone of Inhibition (ZOI) test used well method (three repetitions). The variations in boiling time are 5, 10 and 15 minutes. Inhibition zone measurement using OptiLab version 1.5 software application. Data analysis used One Way ANOVA test with a significance of p <0.05.

Results: The ZOI diameter at kombucha Annona muricata Linn and Clitoria ternatea with 15 minutes of boiling time got the highest average inhibition zone of 14,52±1,7 mm in Escherichia coli but not greater than gentamicin which was 22,43±0,3 mm.

Conclusion: Kombucha Annona muricata Linn and Clitoria ternatea with variations in boiling time of 5,10 and 15 minutes had strong antibacterial potential but lower than gentamicin.

Keywords: Kombucha, Annona muricata L, Clitoria ternatea, Escherichia coli.

 

*Corresponding Author:

Dr.dr. Dini Sri Damayanti, M.Kes

Jl. MT Haryono 193 Malang, Jawa Timur, Indonesia, 65145

Telp. +62341- 558959

e-mail: dinisridamayanti@unisma.ac.id